topoisomerase 3-alpha as a potential drug target and biomarker

Target Name: TOP3A

NCBI ID: G7156

TOP3A

topoisomerase 3-alpha as a potential drug target and biomarker

Topoisomerase 3-alpha (TOP) is an enzyme involved in DNA replication and repair. It is a crucial enzyme for maintaining the integrity of DNA in the cell and plays a key role in preventing genetic mutations. The activity of TOP is regulated by various factors, including temperature, pH, and nucleotide concentrations. Despite its importance, little is known about the structure and function of this enzyme, and it is often used as a protein marker for other cellular processes.

Recent studies have identified potential drug targets and biomarkers for TOP. In this article, we will explore the potential of topoisomerase 3-alpha as a drug target and biomarker, with a focus on its structure, function, and potential therapeutic applications.

Structure and Function

The structure of topoisomerase 3-alpha (TOP) is well conserved among different species, and its primary structure is similar to that of other topoisomerases. It consists of a 21-kDa protein that contains a catalytic domain, a 尾-sheet, and a 纬-hinge. The protein has a molecular weight of 42 kDa and a calculated pI of 6.9.

TOP is regulated by various factors, including temperature, pH, and nucleotide concentrations. At normal temperatures,TOP is active and catalyzes DNA replication in eukaryotic cells. At higher temperatures,TOP is inactive, and DNA replication is blocked. At pH values below 7,TOP is less stable and has reduced catalytic activity. At pH values above 7,TOP becomes more stable and has increased catalytic activity. Additionally, nucleotide concentrations have a significant impact onTOP activity, with higher concentrations leading to increased catalytic activity.

Despite its importance, little is known about the structure and function ofTOP. The primary function ofTOP is to replicate DNA, but it also has been shown to play a role in other cellular processes, including cell signaling, apoptosis, and DNA damage repair.

Potential Therapeutic Applications

The potential therapeutic applications of topoisomerase 3-alpha (TOP) are vast and varied. As a drug target,TOP has the potential to treat a variety of diseases, including cancer, where DNA replication errors can lead to the development of cancer cells. In addition,TOP has been shown to play a role in cell signaling, and alterations in its activity have been observed in various diseases, including neurodegenerative disorders, diabetes, and hypertension.

TOP has also been shown to be a potential biomarker for various diseases. For example, increased TOP activity has been observed in various types of cancer, including breast, ovarian, and prostate cancer. In addition,TOP has been shown to be elevated in individuals with neurodegenerative disorders, such as Alzheimer's disease and Parkinson's disease.

Conclusion

In conclusion, topoisomerase 3-alpha (TOP) is an important enzyme involved in DNA replication and repair. Its structure and function are well conserved among different species, and it has been shown to play a role in various cellular processes. The potential therapeutic applications of TOP are vast and varied, including the treatment of cancer and the detection of various diseases. Further research is needed to fully understand the role ofTOP in cellular processes and its potential as a drug target and biomarker.

Protein Name: DNA Topoisomerase III Alpha

Functions: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. As an essential component of the RMI complex it is involved in chromosome separation and the processing of homologous recombination intermediates to limit DNA crossover formation in cells. Has DNA decatenation activity (PubMed:30057030). It is required for mtDNA decatenation and segregation after completion of replication, in a process that does not require BLM, RMI1 and RMI2 (PubMed:29290614)

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•   expression level;
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