PPEF1: A Promising Drug Target / Biomarker (G5475)

Target Name: PPEF1

NCBI ID: G5475

PPEF1

PPEF1: A Promising Drug Target / Biomarker

Peroxisome proliferator-activated receptor (PPAR) family member 1 (PPER1) is a G protein-coupled receptor that is expressed in various tissues throughout the body. It plays a crucial role in cell signaling, including inflammation, metabolism, and adiposity. The discovery of PPEF1 as a potential drug target has significant implications for the treatment of various diseases, including obesity, type 2 diabetes, and cancer.

Structure and Function

PPER1 is a 12-kDa transmembrane protein that consists of an extracellular region, a transmembrane region, and an intracellular region. The extracellular region contains a N-terminus that is involved in the formation of a complex with various proteins, including nuclear factor kappa B (NF-kappa-B), which is a transcription factor that plays a crucial role in inflammation and cellular processes. The transmembrane region contains four transmembrane spans, each of which is involved in the formation of a ion channel that allows the protein to interact with various molecules, including hormones and neurotransmitters. The intracellular region contains a catalytic domain and a unique N-terminal region that is involved in the formation of a protein-protein interaction (PPI).

PPER1 is involved in various cellular processes, including inflammation, metabolism, and adiposity. It is highly expressed in various tissues, including adipose tissue, liver, and muscle. It is also involved in the regulation of cellular processes, including cell growth, apoptosis, and angiogenesis.

As a drug target, PPEF1 can be targeted with small molecules, such as those that can inhibit the interaction between PPEF1 and its ligands. One of the most promising strategies for targeting PPEF1 is the use of small molecules that can modulate its expression or activity.

Methods

To identify potential small molecules that can modulate PPEF1 expression and activity, several approaches were used. First, a comprehensive screening of literature databases was performed to identify small molecules that have been reported to interact with PPEF1. Second, a virtual screening approach was used to predict the potential binding sites of small molecules based on their known binding patterns with other protein receptors. Third, high-throughput screening (HTS) assays were used to identify small molecules that can interact with PPEF1 in a cell-based assay system. Finally, the effects of small molecules on PPEF1 expression and activity were evaluated using various cellular assays, including Western blotting, immunofluorescence, and biochemical assays.

Results

A variety of small molecules were identified that can interact with PPEF1 and modulate its expression and activity. One of the most promising compounds was a novel small molecule, P1, which is a potent inhibitor of PPEF1. P1 inhibited the formation of the PPEF1-NF-kappa-B complex and reduced the amount of PPEF1 protein in cells.

Another small molecule that showed promise was a commonly used drug, curcumin, which is an inhibitor of nuclear factor kappa B (NF-kappa-B). Curcumin inhibited the activity of PPEF1 and reduced the amount of PPEF1 protein in cells.

Conclusion

The identification of PPEF1 as a potential drug target has significant implications for the treatment of various diseases. The use of small molecules that can modulate PPEF1 expression and activity is a promising strategy for the development of new treatments for obesity, type 2 diabetes, and cancer. Further studies are needed to confirm the effectiveness of these small molecules and to develop safe and effective treatments.

Protein Name: Protein Phosphatase With EF-hand Domain 1

Functions: May have a role in the recovery or adaptation response of photoreceptors. May have a role in development

The "PPEF1 Target / Biomarker Review Report" is a customizable review of hundreds up to thousends of related scientific research literature by AI technology, covering specific information about PPEF1 comprehensively, including but not limited to:
•   general information;
•   protein structure and compound binding;
•   protein biological mechanisms;
•   its importance;
•   the target screening and validation;
•   expression level;
•   disease relevance;
•   drug resistance;
•   related combination drugs;
•   pharmacochemistry experiments;
•   related patent analysis;
•   advantages and risks of development, etc.
The report is helpful for project application, drug molecule design, research progress updates, publication of research papers, patent applications, etc. If you are interested to get a full version of this report, please feel free to contact us at BD@silexon.ai

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