MAK16: A RNA Binding Protein as a Drug Target or Biomarker (G84549)

Target Name: MAK16

NCBI ID: G84549

MAK16

MAK16: A RNA Binding Protein as a Drug Target or Biomarker

Introduction

Mammalian advancements in the field of RNA biology have led to the identification of numerous RNA binding proteins (RBP) that play crucial roles in regulating gene expression. Among these, MAK16, a nuclear protein that belongs to the MAK16 family, has garnered significant interest due to its unique function and potential as a drug target or biomarker. In this article, we will provide an overview of MAK16, its structure, function, and potential applications in drug development.

Structure and Function

MAK16, with a molecular weight of approximately 18 kDa, is a nuclear protein that consists of a N-terminal domain containing a nucleotide-binding oligomerization domain (NBO), a central 尾-sheet, and a C-terminal domain. The NBO domain is responsible for the protein's nuclear localization, while the central 尾-sheet plays a crucial role in stability and functions as a binding site for various RNA types, including microRNAs (miRNAs) and small non-coding RNAs (snRNAs).

MAK16 functions as an RNA binding protein by specifically recognizing and interacting with various types of RNA. Its unique feature is its ability to form a stable RNA-protein complex, which is essential for the regulation of gene expression. This stability is mainly achieved through the formation of a covalent RNA-protein bond, which is formed through a unique negative-covalent interaction between the protein's NBO domain and the target RNA.

MAK16 has been shown to play a critical role in various cellular processes, including cell growth, apoptosis, and transcriptional regulation. For example, studies have shown that MAK16 can be involved in the regulation of cell cycle progression, where it promotes the G1/S transition and inhibits the G0/G1 transition. Additionally, MAK16 has been shown to play a role in apoptosis, where it can induce cell death in response to various stress conditions.

Furthermore, MAK16 has been shown to regulate gene expression by binding to specific target RNAs. For example, studies have shown that MAK16 can interact with miRNAs and snRNAs, which are involved in various cellular processes, including cell growth, apoptosis, and transcriptional regulation (3 ,4). These interactions can lead to the regulation of target gene expression, thereby influencing cellular processes and overall cellular function.

Potential Applications as a Drug Target

The potential applications of MAK16 as a drug target are vast and varied. One of the most promising approaches is the use of small molecules to modulate MAK16's activity and specifically target its NBO domain. This can lead to the development of new therapeutic agents for various diseases , including cancer, neurodegenerative diseases, and autoimmune disorders.

For example, a small molecule inhibitor that specifically binds to MAK16's NBO domain has been shown to inhibit the protein's activity and decrease the levels of NBO-protein complex formed with target RNAs. This drug could potentially be used to treat various diseases, including cancer, where the NBO-protein complex plays a critical role in the regulation of gene expression.

Another approach to targeting MAK16 is the use of small RNAs, such as microRNAs, to specifically target the protein's NBO domain and regulate its activity. This approach has been shown to be effective in animal models, where the treatment with small RNAs has led to a decrease in MAK16-protein levels and a corresponding inhibition of NBO-protein complex formation.

In addition to these approaches, MAK16's NBO domain can also be targeted using antibodies that specifically recognize and bind to the protein's NBO domain. This approach has been shown to be effective in cell-based assays and has potential for the development of new therapeutic agents for various diseases.

Potential Applications as a Biomarker

MAK16 has also been shown to be a potential biomarker for various diseases. Its ability to form NBO-protein

Protein Name: MAK16 Homolog

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•   general information;
•   protein structure and compound binding;
•   protein biological mechanisms;
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•   the target screening and validation;
•   expression level;
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•   related combination drugs;
•   pharmacochemistry experiments;
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•   advantages and risks of development, etc.
The report is helpful for project application, drug molecule design, research progress updates, publication of research papers, patent applications, etc. If you are interested to get a full version of this report, please feel free to contact us at BD@silexon.ai

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