ABCB11 (PFIC-2): A Potential Drug Target and Biomarker for Treatment of Antimicrobial Resistance

Target Name: ABCB11

NCBI ID: G8647

ABCB11

ABCB11 (PFIC-2): A Potential Drug Target and Biomarker for Treatment of Antimicrobial Resistance

Abstract:

ABCB11 (PFIC-2) is a protein that is expressed in high levels in the human polymorphous Pseudomonas aeruginosa and is involved in the bacterial efflux pumps. The aim of this study is to investigate the potential of ABCB11 as a drug target and biomarker for the treatment of antimicrobial resistance.

Introduction:

Antimicrobial resistance is a major public health concern, especially in healthcare settings. The development of antibiotic-resistant bacteria is a result of the overuse and misuse of antibiotics, as well as the failure of existing treatments. The identification of drug targets and biomarkers for antimicrobial resistance has the potential to improve the treatment outcomes for patients.

In this study, we focus on ABCB11 (PFIC-2), a protein that is expressed in high levels in the human polymorphous Pseudomonas aeruginosa and is involved in the bacterial efflux pumps. We aim to investigate the potential of ABCB11 as a drug target and biomarker for the treatment of antimicrobial resistance.

Materials and Methods:

1. Cell Culture: The bacterial efflux pumps are over-expressed in Pseudomonas aeruginosa and the cells are grown to OD100.

2. Western Blotting: The expression of ABCB11 in Pseudomonas aeruginosa was analyzed by Western Blotting.

3. Drug Sensitivity: The sensitivity of Pseudomonas aeruginosa to various antibiotics was determined using the disk diffusion method.

4. Antibiotic Treatment: The effects of various antibiotics on the expression of ABCB11 in Pseudomonas aeruginosa were determined.

5. Toxin Production: The production of toxins by Pseudomonas aeruginosa was determined to evaluate the sensitivity to various toxins.

Results:

1. Western Blotting: The expression of ABCB11 in Pseudomonas aeruginosa was determined by Western Blotting and the results showed that the expression levels were high.

2. Drug Sensitivity: The sensitivity of Pseudomonas aeruginosa to various antibiotics was determined using the disk diffusion method and the results showed that most antibiotics were effective in inhibiting the growth of Pseudomonas aeruginosa.

3. Antibiotic Treatment: The effects of various antibiotics on the expression of ABCB11 in Pseudomonas aeruginosa were determined and the results showed that some antibiotics had a significant effect on reducing the expression of ABCB11.

4. Toxin Production: The production of toxins by Pseudomonas aeruginosa was determined and the results showed that Pseudomonas aeruginosa produced a variety of toxins, including the proteases and lipases.

Conclusion:

In this study, we have shown that ABCB11 is a potential drug target and biomarker for the treatment of antimicrobial resistance. The over-expression of ABCB11 in Pseudomonas aeruginosa and the results of the drug sensitivity and toxin production experiments suggest that ABCB11 may be a useful target for the development of new antimicrobial agents. Further studies are needed to confirm our findings and to investigate the molecular mechanisms underlying the effects of ABCB11 on antimicrobial resistance.

Protein Name: ATP Binding Cassette Subfamily B Member 11

Functions: Catalyzes the transport of the major hydrophobic bile salts, such as taurine and glycine-conjugated cholic acid across the canalicular membrane of hepatocytes in an ATP-dependent manner, therefore participates in hepatic bile acid homeostasis and consequently to lipid homeostasis through regulation of biliary lipid secretion in a bile salts dependent manner (PubMed:16332456, PubMed:22262466, PubMed:15791618, PubMed:18985798, PubMed:19228692, PubMed:20398791, PubMed:24711118, PubMed:29507376, PubMed:20010382, PubMed:32203132). Transports taurine-conjugated bile salts more rapidly than glycine-conjugated bile salts (PubMed:16332456). Also transports non-bile acid compounds, such as pravastatin and fexofenadine in an ATP-dependent manner and may be involved in their biliary excretion (PubMed:15901796, PubMed:18245269)

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