Identification and Characterization of A Potent Inhibitor of NT5DC3

Target Name: NT5DC3

NCBI ID: G51559

NT5DC3

Identification and Characterization of A Potent Inhibitor of NT5DC3

Nucleotidase is an enzyme that plays a crucial role in the DNA replication process. It is a protein that catalyzes the splitting of DNA double-strands into two single-strands during the process of transcription. There are different types of nucleotidases, each with distinct functions and localization to specific cellular processes. One of the nucleotidases that has gained significant attention in recent years is the 5'-nucleotidase domain containing 3 (NT5DC3) protein. This protein is a key component of the nucleosome complex, which It is a protein-nucleic acid complex, mainly responsible for gene transcription. Therefore, NT5DC3 is of great significance in studying gene expression and regulation.

Structure and function

NT5DC3 is an approximately 160 amino acid protein that contains a 5'-nucleotidase domain and three non-ribosome-binding helices (secondary structure). The 5'-nucleotidase domain is a key region for NT5DC3 to catalyze DNA strand separation. This domain contains two core domains: D1 and D2. D1 is an 伪-helix containing 11 core acid residues, while D2 is a 尾-helix containing 12 core acid residues. The link between the two core domains is a 尾-coil consisting of a long sequence of amino acids. These structures enable NT5DC3 to efficiently cleave DNA strands in preparation for the DNA replication process.

The function of NT5DC3 mainly depends on the activity of its 5'-nucleotidase domain. At the 5' end of the DNA molecule, NT5DC3 modifies Ser225 in the D2 core domain through phosphorylation, making it phosphorylation active. Phosphorylation modification enables NT5DC3 to recognize and bind to the 5'-end of DNA molecules, thereby providing phosphate groups for DNA replication. At the 3' end of the DNA molecule, NT5DC3 cleaves the DNA strand through the activity of its 5'-nucleotidase domain. These activities are driven by interactions between two core domains in NT5DC3.

biological activity

In recent years, researchers have discovered many biological activities related to NT5DC3. For example, studies have shown that NT5DC3 plays an important role in inhibiting cell proliferation and inducing apoptosis. In addition, NT5DC3 is also closely related to the occurrence and development of some diseases, such as tumors. In these diseases, abnormal gene expression and DNA damage repair response are considered to be the main causes of the disease. Therefore, studying the role of NT5DC3 in disease treatment has important clinical significance.

pharmacological significance

NT5DC3 has great potential as a drug target in drug development. Many researchers have discovered drug molecules related to NT5DC3 and are studying the mechanisms of action of these drugs in depth. These drug molecules include small molecule compounds, macromolecular compounds, and biological agents.

small molecule compounds

Currently, there are many studies exploring NT5DC3 as small molecule drugs. These small molecule compounds usually exert their biological activity by binding to the active site of NT5DC3. For example, researchers have discovered that 5-[2-(2-isopropylphenyl)acetic acid]-NT5DC3 is a potent inhibitor of NT5DC3. In addition, the researchers also discovered a compound called NTC16, which inhibits the cell proliferation activity of NT5DC3 by binding to its active site. These small molecule compounds are of great significance in drug screening and design.

macromolecular compounds

In addition to small molecule compounds, researchers also found many large molecule compounds related to NT5DC3. These macromolecular compounds often exert their effects by mimicking the biological activity of NT5DC3. For example, researchers have found

Protein Name: 5'-nucleotidase Domain Containing 3

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•   general information;
•   protein structure and compound binding;
•   protein biological mechanisms;
•   its importance;
•   the target screening and validation;
•   expression level;
•   disease relevance;
•   drug resistance;
•   related combination drugs;
•   pharmacochemistry experiments;
•   related patent analysis;
•   advantages and risks of development, etc.
The report is helpful for project application, drug molecule design, research progress updates, publication of research papers, patent applications, etc. If you are interested to get a full version of this report, please feel free to contact us at BD@silexon.ai

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